In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Solid-Phase Synthesis of Aza-phthalocyanine-Oligonucleotide Conjugates and Their Evaluation As New Dark Quenchers of Fluorescence, published in 2010-10-31, which mentions a compound: 56413-95-7, mainly applied to DNA fluorescence hybridization fluorescence quenching solid phase, Formula: C6Cl2N4.
Hydrophobic non-aggregating metal-free azaphthalocyanines (AzaPc) of the tetrapyrazinoporphyrazine type were synthesized, characterized, and used for oligonucleotide labeling. Both 3′-end and 5′-end labeling methods using solid phase synthesis suitable for automatic processes in the DNA/RNA synthesizer were developed. The hydrophobic character of AzaPc enabled the anchoring of the conjugates on reverse phase of the oligonucleotide purification cartridge, thus enabling their simple purification AzaPc did not show any fluorescence and extremely low singlet oxygen quantum yields (ΦΔ = 0.015-0.018 in DMF) in a monomeric state due to ultrafast intramol. charge transfer. That is why they were investigated as a new dark quencher structural type. They profit particularly from absorption in a wide range of wavelengths (300-740 nm) that covers all fluorophores used in hybridization assays nowadays. As an example, quenching efficiency was evaluated in a simple hybridization assay using monolabeled probes. AzaPc-based probes efficiently quenched both fluorescein and Cy5 fluorescence by both resonance energy transfer and contact quenching. The results were compared with three established dark quenchers, and the AzaPc exerted better (BHQ-1 and BHQ-2) or comparable (BBQ-650) quenching efficiencies for both fluorophores.
Here is a brief introduction to this compound(56413-95-7)Formula: C6Cl2N4, if you want to know about other compounds related to this compound(56413-95-7), you can read my other articles.
Reference:
Synthesis and Crystal Structure of a Chiral C3-Symmetric Oxygen Tripodal Ligand and Its Applications to Asymmetric Catalysis,
Chiral lanthanide(III) complexes of sulphur–nitrogen–oxygen ligand derived from aminothiourea and sodium D-camphor-β-sulfonate