Name: 5,6-Dichloropyrazine-2,3-dicarbonitrile. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 5,6-Dichloropyrazine-2,3-dicarbonitrile, is researched, Molecular C6Cl2N4, CAS is 56413-95-7, about Synthesis of new azaphthalocyanine dark quencher and evaluation of its quenching efficiency with different fluorophores. Author is Kopecky, Kamil; Novakova, Veronika; Miletin, Miroslav; Kucera, Radim; Zimcik, Petr.
A new unsym. zinc azaphthalocyanine (AzaPc) was synthesized using statistical condensation of two precursors. Postsynthetic modifications led to incorporation of the azide group that efficiently underwent Cu(I)-catalyzed azide/alkyne 1,3-dipolar cycloaddition with terminal alkyne on a solid phase. The modified solid phase was then used for synthesis of oligodeoxyribonucleotides labeled with AzaPc. DNA hybridization assays confirmed high quenching efficiency (QE>96%) of zinc AzaPc quencher with six different fluorophores ranging in emission maxima from 517 nm to 701 nm (FAM, HEX, Cy3, Cy3.5, Cy5, and Cy5.5).
Although many compounds look similar to this compound(56413-95-7)Name: 5,6-Dichloropyrazine-2,3-dicarbonitrile, numerous studies have shown that this compound(SMILES:N#CC1=NC(Cl)=C(Cl)N=C1C#N), has unique advantages. If you want to know more about similar compounds, you can read my other articles.
Reference:
Synthesis and Crystal Structure of a Chiral C3-Symmetric Oxygen Tripodal Ligand and Its Applications to Asymmetric Catalysis,
Chiral lanthanide(III) complexes of sulphur–nitrogen–oxygen ligand derived from aminothiourea and sodium D-camphor-β-sulfonate